Iranian Journal of Virology
مجله ویروس شناسی ایران
Iran J Virol
Medical Sciences
http://journal.isv.org.ir
1
admin
1735-5680
2588-5030
10.21859/isv
en
jalali
1399
9
1
gregorian
2020
12
1
14
2
online
1
fulltext
en
Molecular Detection and Characterization of Human Adenoviruses in Wastewater and Air Samples of Aeration Tanks
تخصصي
Special
پژوهشي
Original article
<div style="border-bottom:solid windowtext 1.0pt; border-top:solid windowtext 1.0pt; border-left:none; border-right:none; padding:1.0pt 0in 1.0pt 0in"><span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Background and Aims: </span></b><span style="font-size:11.0pt">Human adenoviruses (HAdV) are worldwide distributed pathogens that cause a variety of illnesses, including gastrointestinal infections. These viruses are considered as important pathogens in wastewater, reclamation, and reused water. We aimed to investigate the molecular prevalence and characterization of HAdV in wastewater samples as well as air samples of aeration tanks of a wastewater treatment plant (WWTP) in Tehran, Iran.</span></span></span></span><br>
<span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Materials and Methods: </span></b><span style="font-size:11.0pt">From November 2017 to April 2018, a total of 14 wastewater and 9 air samples were collected and analyzed for the presence of HAdV by an integrated cell culture/polymerase chain reaction (ICC/PCR) technique. The samples were collected from the Ekbatan WWTP in the west of Tehran. To capture bio-aerosols, a liquid impingement biosampler was used. Typing of HAdV was performed by sequencing analysis.</span></span></span></span><br>
<span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Results:</span></b> <span style="font-size:11.0pt">Out of nine untreated</span> <span style="font-size:11.0pt">wastewater samples, one sample (11.1%) was positive for the presence of HAdV cytopathic effect (CPE), while two (40.4%) of five treated wastewater samples were positive for the HAdV CPE. The results of PCR assay also showed that 44.4 % (four out of 9 samples)</span> <span style="font-size:11.0pt">of the untreated wastewater and 60% (three out of 5 samples)</span> <span style="font-size:11.0pt">of the treated wastewater samples</span> <span style="font-size:11.0pt">were positive for HAdV genome. Regarding the air samples</span> <span style="font-size:11.0pt">of aeration tanks, four (44.4%) of nine samples were positive for the presence of the HAdV genome.</span> <span style="font-size:11.0pt">HAdV</span> <span style="font-size:11.0pt">in the wastewater samples were type B (40%), type C (40%), and type D (20%) and all four positive air samples for HAdV were type C</span><span style="font-size:11.0pt">.</span></span></span></span><br>
<span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Conclusion: </span></b><span style="font-size:11.0pt">The human adenovirus detected in 50% of the wastewater and 44.44% of the air samples of the wastewater treatment plant of the Ekbatan, Tehran, Iran</span><span style="font-size:11.0pt">.</span></span></span></span></div>
Human adenovirus, Waste Water, Molecular typing
38
43
http://journal.isv.org.ir/browse.php?a_code=A-10-1-102&slc_lang=en&sid=1
G
Mokhtary-Irani
10031947532846007183
10031947532846007183
No
Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
A
Tavakoli
10031947532846007184
10031947532846007184
No
Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran. University of Medical Sciences, Tehran, Iran.
F
Bokharaei-Salim
10031947532846007185
10031947532846007185
No
Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
M
Farzadkia
10031947532846007186
10031947532846007186
No
Department of Environmental Health Engineering, Faculty of Public Health, Iran University of Medical Sciences, Tehran, Iran.
A
Tabibzadeh
10031947532846007187
10031947532846007187
No
Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
SJ
Kiani
10031947532846007188
10031947532846007188
No
Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
M
Esghaei
10031947532846007189
10031947532846007189
No
Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
SH
, Monavari
10031947532846007190
10031947532846007190
No
Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
D
Javanmard
10031947532846007191
10031947532846007191
No
Infectious Diseases Research Center, Birjand University of Medical science, Birjand, Iran.
Z
Azarash
10031947532846007192
10031947532846007192
No
Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
A
Kachooei
10031947532846007193
10031947532846007193
No
Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
A
Ataei-Pirkooh
10031947532846007194
10031947532846007194
Yes
Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.