en تخصصي Special پژوهشي Original article <div style="border-bottom:solid windowtext 1.0pt; border-top:solid windowtext 1.0pt; border-left:none; border-right:none; padding:1.0pt 0in 1.0pt 0in"><span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-size:11.0pt">Foot-and-mouth disease virus (FMDV) causes severe infection in livestock and makes considerable economic impacts. Therefore, a rapid, highly specific, and accurate method for the diagnosis of FMDV infections is required to ensure that appropriate treatment is administered to reduce economic losses. In this study, the diagnostic tests for FMDV detection by enzyme-linked immunosorbent (Ag-ELISA), reverse transcription&ndash;PCR (RT-PCR), and real-time RT-PCR (rRT-PCR) were carried out as the World Organization for Animal Health recommended and were based on the VP1 gene. Positive samples were detected by RT-PCR and rRT-PCR (73.16%) and by ELISA test (55.99%). According to the information obtained from the present study molecular methods provide much more reliable and definitive results than Immune assay methods.</span></span></span></span></div> <br> &nbsp; FMD virus, ELISA, RT-PCR, rRT-PCR 57 63 http://journal.isv.org.ir/browse.php?a_code=A-10-320-1&slc_lang=en&sid=1 Fatemeh Sadat Mousavi Fatememousavi7715@gmail.com 10031947532846007614 10031947532846007614 No Department of Virology, Faculty of veterinary Medicine, University of Tehran, Tehran, Iran Bahman Abedi Kiasari abedikiasari.b@ut.ac.ir 10031947532846007615 10031947532846007615 Yes Microbiology and Immunology Group, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Ali Reza Khosravi Khosravi@ut.ac.ir 10031947532846007616 10031947532846007616 No Microbiology and Immunology Group, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Akbar Khorasani akbarkhorasani@gmail.com 10031947532846007617 10031947532846007617 No Foot and Mouth Disease Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, IR Iran Mohammad Kazem Koohi mkkoohi@ut.ac.ir 10031947532846007618 10031947532846007618 No Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Mohammad Mehdi Ranjbar mm.ranjbar.phd@gmail.com 10031947532846007619 10031947532846007619 No Foot and Mouth Disease Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, IR Iran Ali Reza Yousefi rezayousefi2005@gmail.com 10031947532846007620 10031947532846007620 No Foot and Mouth Disease Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, IR Iran Homayoon Mahravani H.mahravani@vsri.ac.ir 10031947532846007621 10031947532846007621 No Foot and Mouth Disease Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, IR Iran Sanaz Majidi majidisanaz69@gmail.com 10031947532846007622 10031947532846007622 No Department of Virology, Faculty of veterinary Medicine, University of Tehran, Tehran, Iran Mojtaba Ranji Mojtabaranji313@gmail.com 10031947532846007623 10031947532846007623 No Foot and Mouth Disease Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, IR Iran