Iranian Journal of Virology

en Molecular Epidemiology of Caprine Arthritis Encephalitis Virus (CAEV) in Imported and Indigenous Dairy Goat Breeds in Iran تخصصي Special پژوهشي Original article <div style="border-bottom:solid windowtext 1.0pt; border-top:solid windowtext 1.0pt; border-left:none; border-right:none; padding:1.0pt 0in 1.0pt 0in">Abstract Background and Aims: Virus of caprine arthritis encephalitis belongs to the Retrovirdae family, which can cause arthritis, mastitis and abortion in adult goats, and encephalitis in kids especially in dairy breeds. Therfore, income of farmers are affected .since, in the recent years many importation of small ruminant animals especially dairy goats breeds to our country have been done . This study was conducted to evaluate infection of CAEV in imported and indigenous dairy goat breeds which were kept together in one herd. Materials and methods: In this study, blood samples were taken from 249 dairy goats in one of the industrial breeding farm. Buffy coat was separated from the samples using centrifugation and DNA was extracted from them. Nested polymerase chain reaction (Nested PCR) method was performed with specific primers in order to detect the gag gene. Results: The gag gene was detected in21 blood samples (8.3%) out of 249  samples and result of CAEV  Infection rate was 11 (13.8%), 7 (8.1%) and 3 (3.6%) for Alpine, Saanen and Indigenous breeds, respectively. Percent of infection based on old was 15.8%, in < two years goats, 8% in 2 3 years and 7.7% > three years old. Conclusion: Unfortunately, the imported and local breed of dairy goats were infected with CAEV, therefore, phylogeny studies are required to for identification of prevalence of the disease in herds of the country.</div> Caprine arthritis encephalitis, CAEV, Nested PCR, gag, Iran 63 69 http://journal.isv.org.ir/browse.php?a_code=A-10-348-1&slc_lang=en&sid=1 Fereydoun Mansouri manesht_1100@yahoo.com 10031947532846007869 10031947532846007869 No Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Bahman Abedi kiasari abedikiasari.b@ut.ac.ir 10031947532846007870 10031947532846007870 Yes Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Mahdi Vejgani vodjgani@ut.ac.ir 10031947532846007871 10031947532846007871 No Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Mohammad Mehdi Ranjbar mm.ranjbar.phd@gmail.com 10031947532846007872 10031947532846007872 No Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran. Shahab Falahi Shahabivan@gmail.com 10031947532846007873 10031947532846007873 No Zoonotic Diseases Research Center, Ilam University of Medical Sciences, Ilam, Iran