en عمومى General پژوهشي Original article Background and Aims: The aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcDNA3.1(+) in BSR cell line. This construct might be used for a potential DNA vaccine. Materials and Methods: Glycoprotein gene was synthesized and cloned into pBluescript vector and then sub cloned into eukaryotic expression vector (pcDNA3.1(+)). After verification of the cloning, the recombinant plasmid was transfected into BSR cell line (a clone of BHK-21 cell), and its expression was detected by RT-PCR. Results: The authenticity of the recombinant plasmid pcDNA3.1(+)-Gp has been confirmed by a quick check method and restriction endonuclease digestion analysis, and after transfection into eukaryotic cells, the presence of mRNA transcripts was verified by reverse transcriptase-polymerase chain reaction (RT-PCR). Conclusion: This study demonstrated that the construction of eukaryotic expression plasmid for rabies virus glycoprotein is possible. Nevertheless, more work is necessary to develop this kind of vaccine for final use. Rabies virus, glycoprotein, DNA vaccine, Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) 8 11 http://journal.isv.org.ir/browse.php?a_code=A-10-1-67&slc_lang=en&sid=1 K Borhani 10031947532846003540 10031947532846003540 No Department of Virology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran T Bamdad 10031947532846003541 10031947532846003541 Yes Department of Virology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran M Ajorloo 10031947532846003542 10031947532846003542 No Department of Virology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran SHR Mozhgani 10031947532846003543 10031947532846003543 No Human Rabies Vaccine Laboratory, Production and Research Complex Pasteur, Institute of Iran, Tehran, Iran N Miandehi 10031947532846003544 10031947532846003544 No Viral Vaccines Production Department, Production and Research Complex Pasteur, Institute of Iran, Tehran, Iran A Moradi-Joshaghan 10031947532846003545 10031947532846003545 No Viral Vaccines Production Department, Production and Research Complex Pasteur, Institute of Iran, Tehran, Iran AR Gholami 10031947532846003546 10031947532846003546 No Viral Vaccines Production Department, Production and Research Complex Pasteur, Institute of Iran, Tehran, Iran