en
jalali
1386
8
1
gregorian
2007
11
1
1
4
online
1
fulltext
en
Reovirus oncolysis: a brief insight on molecular mechanism and immunological aspect
Abstract : Reovirus (respiratory enteric orphan virus), a naturally occurring benign human pathogen, has an inherent ability to target transformed and cancerous cells and cause their lysis, while leaving non-transformed cells relatively unaffected. The efficiency of this innate oncolytic activity of reovirus correlates with expression of the ras oncogene. Cells expressing activated Ras and the related Ras/RalGEF/p38 pathway are more permissive to the reovirus infection than that of untransformed counterparts. Ras-transformation orchestrates selective oncolysis of cancerous cells by mediating efficient virus uncoating as well as by enhancing infectivity and subsequent apoptosis-dependent release of nascent virus particles. Different human and murine cell lines derived from naturally occurring tumors also display similar activation of the ras pathway, and thus present selective susceptibility to reovirus oncolysis under in vitro as well as in vivo conditions. This ability of reovirus to selectively target a wide variety of tumors offers a novel anti-cancer therapeutic option. However, the efficiency of reovirus virotherapy in immunocompetent hosts is compromised due to the presence of anti-viral innate and adaptive immune responses. Hence, the success of this highly promising reovirus oncolytic therapy will likely be enhanced by modulating host immunity.
reovirus , cancer , virotherapy , oncolysis , ras oncogene
1
9
http://journal.isv.org.ir/browse.php?a_code=A-10-43-23&slc_lang=en&sid=1
2016/08/27
1395/6/6
2016/08/27
1395/6/6
S.A
Gujar
Department of Microbiology and Immunology, Dalhousie University Halifax, Nova Scotia, Canada
0031947532846002385
0031947532846002385
Yes
P.W.K
Lee
Department of Microbiology and Immunology, Dalhousie University Halifax, Nova Scotia, Canada
Patrick.lee@dal.ca
0031947532846002386
0031947532846002386
No
en
Peganum harmala seed extract can prevent HSV-1 replication in vitro
Abstract: Peganum harmala L. (Zygophylaceae) has been used in a variety of practical applications in medical science. Our objective in the current study was to determine the effects of the seed extract on Herpes simplex virus type 1 (HSV-1) replication in Vero cells. Different concentrations (100, 500, 571, 667, 800 and 1000 µg/ml) of the extract were examined. Experiments were carried out using Vero cells. P.harmala seed extract was found to be non-toxic to Vero cells up to a concentration of 667 µg/ml. The antiviral activity of non-toxic concentration against HSV-1 was tested. The replication of HSV-1 was inhibited, indicating that the P.harmala L. extract contains an anti-HSV-1 substance.
Peganum harmala L. ,Vero cells ,TCID50 ,Cytotoxicity
11
16
http://journal.isv.org.ir/browse.php?a_code=A-10-43-24&slc_lang=en&sid=1
2016/08/272016/08/27
1395/6/6
2016/08/272016/08/27
1395/6/6
S.J
Kiani
Department of Virology, Iran University of Medical Sciences, Tehran, IRAN
0031947532846002387
0031947532846002387
Yes
M
Shamsi Shahrabadi
Department of Virology, Iran University of Medical Sciences, Tehran, IRAN
Mshahrabadi@hotmail.com
0031947532846002388
0031947532846002388
No
A
Ataei
Department of Virology, Iran University of Medical Sciences, Tehran, IRAN
0031947532846002389
0031947532846002389
No
N
Sajjadi
Department of Virology, Iran University of Medical Sciences, Tehran, IRAN
0031947532846002390
0031947532846002390
No
en
Hepatitis C virus infection and genotypes in blood donors
Abstract: Genotyping of the hepatitis C virus (HCV) is important for designing therapeutic strategies and regional specific diagnostic assays. The aim of this study was to identify the HCV genotypes in HCV infected blood donors. This is the first report on HCV genotypes in blood donors in Iran. In this cross-sectional study, 103 blood donors with hepatitis C were investigated for HCV genotypes. HCV genotyping was carried out using type-specific primers from the core region of the viral genome. From 103 blood donors, only96 cases had genotypes which could be typed. The highest frequency genotype 1a, with 53 (51.5%) of subjects. Genotype 3a and 1b were the other frequent genotypes with 39 (37.9 %) and 4 (3.9%) subjects, respec-tively. These results indicate that the dominant HCV genotypes among blood donors were 1a, 3a and 1b respectively. It was also noticed that more of the blood donors infected with genotypes 1a and 3a had history of intravenous drug abuse and tattooing.
HCV , genotypes , blood donors , Iran
17
22
http://journal.isv.org.ir/browse.php?a_code=A-10-43-25&slc_lang=en&sid=1
2016/08/272016/08/272016/08/27
1395/6/6
2016/08/272016/08/272016/08/27
1395/6/6
Z
Sharifi
Assistant professor of Research center, Iranian Blood Transfusion Organization (IBTO), Tehran, Iran.
z.sharifi@ibto.ir
0031947532846002391
0031947532846002391
Yes
M
Mahmoudian Shooshtari
Assistant professor of Research center, Iranian Blood Transfusion Organization (IBTO), Tehran, Iran.
0031947532846002392
0031947532846002392
No
en
A Reverse transcription-PCR assay for detection of type A influenza virus and differentiation of avian H7 subtype
Abstract : Avian influenza virus (AIV) infection is a major cause of influenza mortality in birds and can cause human mortality and morbidity. Although the risk of infection with avian influenza virus (AIV) is generally low for most people, the pathogenic virus can cross the species barrier and acquires the ability to infect and be transmitted among the human population; therefore the rapid identification of the virus is of important clinical and epidemiological implication. A reverse transcriptase-polymerase chain reaction (RT-PCR) was optimized for the detection of type A influenza virus. The assay differentiates avian H7 hemagglutinin subtypes. Two sets of specific oligonucleotide primers were used in this test for type A influenza virus and H7 heamagglutinin subtypes. The RT-PCR DNA products were visualized by gel electrophoresis and consisted of fragments of 98 bp for H7 hemagglutinin subtypes and 101 bp for type A influenza virus. The common set of primers for type A influenza virus were able to amplify a 101 bp DNA band for any of the other subtypes of influenza A virus. The RT-PCR assay developed in this study was found to be sensitive and specific. No specific amplification bands of the same sizes (98 bp) could be amplified for RNA of other influenza hemagglutinin subtypes, specific amplification bands of type A influenza (101 bp) for Influenza B, C, or other viral or bacterial pathogens was not tested in this study.
Influenza A ,RT-PCR ,Hemagglutinin ,H7 ,Subtyping
23
26
http://journal.isv.org.ir/browse.php?a_code=A-10-43-26&slc_lang=en&sid=1
2016/08/272016/08/272016/08/272016/08/27
1395/6/6
2016/08/272016/08/272016/08/272016/08/27
1395/6/6
E
Saberfar
Research Center of Virus & Vaccine, Baqiyatallah (a.s.) University of Medical Sciences, Tehran, IR Iran.
saberfar @yahoo.com
0031947532846002393
0031947532846002393
Yes
A
Najafi
Research center of molecular biology, Baqiyatallah (a.s.) University of Medical Sciences, Tehran, IR Iran.
0031947532846002394
0031947532846002394
No
H
Lashini
Research Center of Virus & Vaccine, Baqiyatallah (a.s.) University of Medical Sciences, Tehran, IR Iran.
0031947532846002395
0031947532846002395
No
en
Evaluation of immune status to measles in vaccinated population in Tehran, using enzyme-linked immunosorbent assay and the hemagglutination inhibition techniques
Abstract: Measles remains one of the leading causes of childhood morbidity and mortality in developing countries and is still a major public health concern in developed countries. Although live attenuated vaccine is used throughout the world, out breaks of disease still occur in many countries including Iran. Understanding measles outbreaks that occur after the initiation of measles elimination efforts will be critical in refining the strategies for measles elimination. The present study was performed to evaluate the immune status against measles after the mass campaign vaccination in 2003.In this study, Approximately 172 sera were analysed by ELISA and HI tests. The results indicated, 162 were positive (94.2%) and 10 were negative (5.8%) by HI test and 165 were positive (95.5%) and 7 were negative (4.1%) by the ELISA test.
Measles ,immune status ,ELISA ,HI
27
30
http://journal.isv.org.ir/browse.php?a_code=A-10-43-27&slc_lang=en&sid=1
2016/08/272016/08/272016/08/272016/08/272016/08/27
1395/6/6
2016/08/272016/08/272016/08/272016/08/272016/08/27
1395/6/6
M
Fazlalipour
Department of Virology, Iran University of Medical sciences
0031947532846002396
0031947532846002396
Yes
S.H
Monavari
Department of Virology, Iran University of Medical sciences
hrmonavari@yahoo.com
0031947532846002397
0031947532846002397
No
M
Shamsi Shahrabadi
Department of Virology, Iran University of Medical sciences
0031947532846002398
0031947532846002398
No
A
Ataei
Department of Virology, Iran University of Medical sciences
0031947532846002399
0031947532846002399
No
en
Study of biological and molecular characterization of pepper-PVY isolated from Tehran pepper fields and it’s comparison with other PVY isolates
Abstract: Potato virus Y a type species of the genus potyvirus infects several crops in the family solanaceae. PVY isolated from field infected peppers was identified on the basis of host reaction, serological and molecular characterization. The result of ELISA, Immunoblot electrophoresis and Immuno Capture Reverse Transcription Polymerase Chain Reaction (IC-RT-PCR) indicated that pepper isolate of PVY shares the reported properties of the PVY but in host range studies some differences were observed. For this reason the sequence analysis was performed .This is the first report of PVY incidence in Iran pepper fields.
Potato virus Y , IC-RT-PCR , Immuno blot electrophoresis
31
34
http://journal.isv.org.ir/browse.php?a_code=A-10-43-28&slc_lang=en&sid=1
2016/08/272016/08/272016/08/272016/08/272016/08/272016/08/27
1395/6/6
2016/08/272016/08/272016/08/272016/08/272016/08/272016/08/27
1395/6/6
S
Mostafae
College of agriculture and Natural Resources Faculty of Horticulture Sciences and Plant Protection, University of Tehran
saghar_200059@yahoo.com
0031947532846002400
0031947532846002400
Yes
G
Mosahebi
College of agriculture and Natural Resources Faculty of Horticulture Sciences and Plant Protection, University of Tehran
0031947532846002401
0031947532846002401
No
M
Koohi Habibi
College of agriculture and Natural Resources Faculty of Horticulture Sciences and Plant Protection, University of Tehran
0031947532846002402
0031947532846002402
No
E
Ansari.Dezfouli
College of Agriculture, Faculty of Biotechnology, University of Kerman
0031947532846002403
0031947532846002403
No
fa
Differentiation between viral exanthema and allergic exanthema by IFN-γ and IL-4 assay
viral exanthema, allergic exanthema, IFN-γ, IL-4
35
37
http://journal.isv.org.ir/browse.php?a_code=A-10-43-29&slc_lang=en&sid=1
2016/08/272016/08/272016/08/272016/08/272016/08/272016/08/272016/08/27
1395/6/6
2016/08/272016/08/272016/08/272016/08/272016/08/272016/08/272016/08/27
1395/6/6
T
Mokhtari Azad
Dept. of Virology, School of Public Health and Institute of Health Research, Tehran University of Medical Sciences.
Mokhtari @ sina.tums.ac.ir
0031947532846002404
0031947532846002404
Yes
A
Mirzaie
Dept. of Virology, School of Public Health and Institute of Health Research, Tehran University of Medical Sciences.
0031947532846002405
0031947532846002405
No
F
Rezaie
Dept. of Virology, School of Public Health and Institute of Health Research, Tehran University of Medical Sciences.
0031947532846002406
0031947532846002406
No
Z
Seadatmand
Dept. of Virology, School of Public Health and Institute of Health Research, Tehran University of Medical Sciences.
0031947532846002407
0031947532846002407
No
V
Salimi
Dept. of Virology, School of Public Health and Institute of Health Research, Tehran University of Medical Sciences.
0031947532846002408
0031947532846002408
No
R
Hamkar
Dept. of Virology, School of Public Health and Institute of Health Research, Tehran University of Medical Sciences.
0031947532846002409
0031947532846002409
No