[صفحه اصلی ]   [Archive] [ English ]  
:: صفحه اصلي :: درباره نشريه :: آخرين شماره :: تمام شماره‌ها :: جستجو :: ثبت نام :: ارسال مقاله :: تماس با ما ::
:: دوره 7، شماره 1 و 2 - ( 4-1392 ) ::
جلد 7 شماره 1 و 2 صفحات 21-29 برگشت به فهرست نسخه ها
Diagnosis of Foot-and-Mouth Disease Virus by Real Time Reverse Transcription Polymerase Chain Reaction Assay in Iran
چکیده:   (3285 مشاهده)
Background and Aims: Accurate and rapid diagnosis is necessary for effective control and prevention of foot-and-mouth disease (FMD). In present study, was evaluated real time reverse transcription-polymerase chain reaction (rRT-PCR) assay along with diagnostic routine methods for the detection of all seven serotypes of FMD virus (FMDV), namely O, C, A, SAT1, 2, 3 and Asia 1 in biological samples at the reference laboratory for FMD, Iran. Materials and Methods: Two different RT-PCR assays targeting two different regions 5´ untranslated region (5´-UTR) and RNA polymerase (3D) of the FMDV genome were used to confirm the presence of FMDV in epithelial suspensions. Results: In the two methods the viral RNA in all tested archival serotypes of FMDV were detected. Specificity of this reaction was confirmed by the use of swine vesicular disease virus and blue-tongue. The amount of cycle threshold (CT) value of all seven serotypes was different and the lowest and highest of CT value achieved for SAT3, A, O types and SAT2, C types, respectively. Conclusion: The results showed that RT-PCR was more sensitive and effective than routine diagnostic methods. Furthermore, RT-PCR as a strong and valuable tool concomitant with diagnostic routine methods facilitate monitoring the fields FMDV strains and suggested that the use of the multiple diagnostic targets could enhance the sensitivity of the molecular methods for the detection of FMDV.
متن کامل [PDF 440 kb]   (1630 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: عمومى
دریافت: ۱۳۹۳/۹/۱۸ | پذیرش: ۱۳۹۳/۹/۱۸ | انتشار: ۱۳۹۳/۹/۱۸
ارسال نظر درباره این مقاله
نام کاربری یا پست الکترونیک شما:

CAPTCHA



XML   English Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ahmadi-Vasmehjani A, Mousavi-Nasab S, Baharlou R, Jeirani F, Shayestehpour M, Yaghoubi S, et al . Diagnosis of Foot-and-Mouth Disease Virus by Real Time Reverse Transcription Polymerase Chain Reaction Assay in Iran. Iran J Virol. 2013; 7 (1 and 2) :21-29
URL: http://journal.isv.org.ir/article-1-114-fa.html

Diagnosis of Foot-and-Mouth Disease Virus by Real Time Reverse Transcription Polymerase Chain Reaction Assay in Iran. مجله ویروس شناسی ایران. 1392; 7 (1 و 2) :21-29

URL: http://journal.isv.org.ir/article-1-114-fa.html



دوره 7، شماره 1 و 2 - ( 4-1392 ) برگشت به فهرست نسخه ها
Persian site map - English site map - Created in 0.05 seconds with 32 queries by YEKTAWEB 3977