[صفحه اصلی ]   [Archive] [ English ]  
:: صفحه اصلي :: درباره نشريه :: آخرين شماره :: تمام شماره‌ها :: جستجو :: ثبت نام :: ارسال مقاله :: تماس با ما ::
:: دوره 3، شماره 1 - ( 3-1388 ) ::
جلد 3 شماره 1 صفحات 13-17 برگشت به فهرست نسخه ها
Detection of Herpes Simplex Virus Antibodies Using the Whole Virus and Recombinant gD
چکیده:   (3508 مشاهده)

Background and Aims: Herpes simplex virus type 1 (HSV1) remains a potentially serious health problem world wide. All infected people, including asymptomatic ones, are potential sources for virus transmission. Virus envelope contains at least 13 glycoproteins, which glycoprotein D is the major target of immune responses. The aim of this study was development of a specific method that is a more rapid, sensitive and specific test compared to the virus neutralization test which is applied as gold standard test.

Methods: In this study, the Western blot technique using crude HSV1 whole particle and baculovirus derived glycoprotein D of HSV1 as antigens was set. Human sera were analyzed by virus neutralization test and then serum samples with reciprocal virus neutralization antibody titers of 32, 64, and 128 were taken to be analyzed by Western blotting.

Results: It was shown that there was a very good correlation between results obtained from virus neutralization antibody titers and those of Western blotting. Western blotting using recombinant glycoprotein D of HSV1 as an antigen showed positive results similar to the whole HSV1 antigen.

Conclusion: Our study showed the Western blotting using recombinant glycoprotein D can replace virus neutralization test in diagnosis of HSV1 infections.
متن کامل [PDF 133 kb]   (486 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: عمومى
دریافت: ۱۳۹۳/۷/۶ | پذیرش: ۱۳۹۳/۷/۶ | انتشار: ۱۳۹۳/۷/۶
ارسال نظر درباره این مقاله
نام کاربری یا پست الکترونیک شما:

CAPTCHA



XML   English Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Meshkat Z, Soleimanjahi H, Roostaee M, Bamdad T, Meshkat M. Detection of Herpes Simplex Virus Antibodies Using the Whole Virus and Recombinant gD. Iran J Virol. 2009; 3 (1) :13-17
URL: http://journal.isv.org.ir/article-1-23-fa.html

Detection of Herpes Simplex Virus Antibodies Using the Whole Virus and Recombinant gD. مجله ویروس شناسی ایران. 1388; 3 (1) :13-17

URL: http://journal.isv.org.ir/article-1-23-fa.html



دوره 3، شماره 1 - ( 3-1388 ) برگشت به فهرست نسخه ها
Persian site map - English site map - Created in 0.06 seconds with 32 queries by YEKTAWEB 3974