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Molecular Epidemiology of Caprine Arthritis Encephalitis Virus (CAEV) in Imported and Indigenous Dairy Goat Breeds in Iran
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چکیده: (399 مشاهده) |
Abstract
Background and Aims: Virus of caprine arthritis encephalitis belongs to the Retrovirdae family, which can cause arthritis, mastitis and abortion in adult goats, and encephalitis in kids especially in dairy breeds. Therfore, income of farmers are affected .since, in the recent years many importation of small ruminant animals especially dairy goats breeds to our country have been done . This study was conducted to evaluate infection of CAEV in imported and indigenous dairy goat breeds which were kept together in one herd.
Materials and methods: In this study, blood samples were taken from 249 dairy goats in one of the industrial breeding farm. Buffy coat was separated from the samples using centrifugation and DNA was extracted from them. Nested polymerase chain reaction (Nested PCR) method was performed with specific primers in order to detect the gag gene.
Results: The gag gene was detected in21 blood samples (8.3%) out of 249 samples and result of CAEV Infection rate was 11 (13.8%), 7 (8.1%) and 3 (3.6%) for Alpine, Saanen and Indigenous breeds, respectively. Percent of infection based on old was 15.8%, in < two years goats, 8% in 2 3 years and 7.7% > three years old.
Conclusion: Unfortunately, the imported and local breed of dairy goats were infected with CAEV, therefore, phylogeny studies are required to for identification of prevalence of the disease in herds of the country. |
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متن کامل [PDF 539 kb]
(208 دریافت)
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نوع مطالعه: پژوهشي |
موضوع مقاله:
تخصصي
دریافت: 1402/10/26 | پذیرش: 1403/3/31 | انتشار: 1402/5/6
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