[صفحه اصلی ]   [Archive]  
:: صفحه اصلي :: درباره نشريه :: آخرين شماره :: تمام شماره‌ها :: جستجو :: ثبت نام :: ارسال مقاله :: تماس با ما ::
بخش‌های اصلی
آرشیو مجله و مقالات::
برای نویسندگان::
برای داوران::
ثبت نام و اشتراک::
تماس با ما::
تسهیلات پایگاه::
بایگانی مقالات زیر چاپ::
::
جستجو در پایگاه

جستجوی پیشرفته
..
:: دوره 5، شماره 1 - ( 11-1389 ) ::
جلد 5 شماره 1 صفحات 22-15 برگشت به فهرست نسخه ها
Evaluation of Adventitious Agents in MMR & Oral Poliomyelitis Vaccines
چکیده:   (5367 مشاهده)
Background and Aims: Adventitious agents, especially viral agents are among the most important concerns in viral vaccines. Viral contamination of biological products may arise from the original source of the reagents such as, serum, trypsin, animal or human derived media components or cell culture and working seed or cross contamination of vaccine during production. In this study, adventitious agents were evaluated in the MMR and oral polio vaccines. Methods: For detection of adventitious agents, two techniques were used. The suspensions that were harvested after inoculation on MRC-5 were neutralized with specific antisera and inoculated into Vero, HeLa and MRC-5 cells (in vitro tests). The suspensions that harvested after inoculation of CHEF primary cell culture were neutralized with specific antisera and inoculated in to Vero, MRC-5, CHEF, chorioallantoic membrane and yolk sac of SPF embryonated eggs (in vivo tests). Results: As indications of viral contamination, CPE in cell culture and the viability of egg embryo were observed and haemadsorption and haemagglutination test were performed. Finally, data were analyzed by exact binomial test (version 2.8.1). There was not any CPE in cell culture, the inoculated embryos were viable and there was not any haemadsorption & haemagglutination in all of the samples. Conclusion: The major focus of this Study will be to ensure that vaccines are devoid of adventitious agents. There were not any signs of viral agents in the samples and the preparations could be used for vaccine production.
متن کامل [PDF 349 kb]   (2120 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: عمومى
دریافت: 1393/8/16 | پذیرش: 1393/8/16 | انتشار: 1393/8/16
ارسال نظر درباره این مقاله
نام کاربری یا پست الکترونیک شما:

CAPTCHA



XML   English Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Soleimani S, Lotfi M, Taghavian M, Shahkarami M. Evaluation of Adventitious Agents in MMR & Oral Poliomyelitis Vaccines. Iran J Virol 2011; 5 (1) :15-22
URL: http://journal.isv.org.ir/article-1-62-fa.html

Evaluation of Adventitious Agents in MMR & Oral Poliomyelitis Vaccines. مجله ویروس شناسی ایران. 1389; 5 (1) :15-22

URL: http://journal.isv.org.ir/article-1-62-fa.html



بازنشر اطلاعات
Creative Commons License این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است.
دوره 5، شماره 1 - ( 11-1389 ) برگشت به فهرست نسخه ها
Persian site map - English site map - Created in 0.05 seconds with 37 queries by YEKTAWEB 4645