:: دوره 11، شماره 2 - ( 3-1396 ) ::
جلد 11 شماره 2 صفحات 14-18 برگشت به فهرست نسخه ها
Identification of RD and L20B Cell Lines Cross-Contamination by Multiplex SYBR-Green PCR in Poliovirus Laboratory
چکیده:   (1572 مشاهده)
Background and Aims: World Health Organization Global Polio Laboratory Network (GPLN) plays a critical role in the Global Polio Eradication Initiative. Cell culture methods (mostly RD and L20B cell lines) have been used for Enteroviruses and polioviruses isolation, respectively. Cross-contamination among L20B and RD cell lines causes the problem in accuracy of poliovirus surveillance and decreases the poliovirus detection. Therefore, validation of identity of cell lines purity is a vital part of cell culture in polio laboratory.
Materials and Methods: In this study, a multiplex SYBR-Green PCR based on Cytochrome b oxidase amplification was designed to L20B and RD cell lines cross-contamination. Results: The conventional multiplex PCR performed on DNA extracted from L20B cells deliberately cross-contaminated with RD cells clearly showed not only the identity of L20B cell line but also the presence of contaminant RD cells.
Conclusions: The results indicated that the multiplex SYBR-Green PCR was reliable method to identity L20B and RD cell lines individually and also after deliberate cross-contamination.
متن کامل [PDF 504 kb]   (790 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: عمومى
دریافت: 1396/9/12 | پذیرش: 1397/1/26 | انتشار: 1397/1/26


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دوره 11، شماره 2 - ( 3-1396 ) برگشت به فهرست نسخه ها