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Comparison of Immune Assay and Molecular Methods For Diagnosis Of FMD Virus
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Fatemeh Sadat Mousavi   , Bahman Abedi Kiasari *  , Ali Reza Khosravi , Akbar Khorasani , Mohammad Kazem Koohi , Mohammad Mehdi Ranjbar , Ali Reza Yousefi , Homayoon Mahravani , Sanaz Majidi , Mojtaba Ranji |
| Microbiology and Immunology Group, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. |
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Abstract: (465 Views) |
Foot-and-mouth disease virus (FMDV) causes severe infection in livestock and makes considerable economic impacts. Therefore, a rapid, highly specific, and accurate method for the diagnosis of FMDV infections is required to ensure that appropriate treatment is administered to reduce economic losses. In this study, the diagnostic tests for FMDV detection by enzyme-linked immunosorbent (Ag-ELISA), reverse transcription–PCR (RT-PCR), and real-time RT-PCR (rRT-PCR) were carried out as the World Organization for Animal Health recommended and were based on the VP1 gene. Positive samples were detected by RT-PCR and rRT-PCR (73.16%) and by ELISA test (55.99%). According to the information obtained from the present study molecular methods provide much more reliable and definitive results than Immune assay methods.
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| Keywords: FMD virus, ELISA, RT-PCR, rRT-PCR |
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Full-Text [PDF 754 kb]
(80 Downloads)
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Type of Study: Original article |
Subject:
Special
Received: 2022/12/18 | Accepted: 2022/12/19 | Published: 2022/12/19
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