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:: Volume 7, Issue 3 (9-2013) ::
Iran J Virol 2013, 7(3): 22-26 Back to browse issues page
Adenovector Mediated Inflammation in Mice Liver Boosted by Hydrodynamic Injection
Fatemeh Rezaei, Seyed Younes Hosseini *, Ameneh Pakniat, Bit Geramizadeh, Mohammadreza Fattahi, Narges Nejabat, Farhad Koohpeyma
GastroenteroHepatology Research center, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract:   (3968 Views)
Background and Aims: Adenovector gene transfer induces inflammatory response that finally leads to vector removal from delivered site. The effect of hydrodynamic pre-injection on Adenovector mediated liver inflammation remained elusive. Materials and Methods: Different mice groups were pre-treated by hydrodynamic saline, dexamethasone or nothing prior to Adeno-Luciferase administration. Their samples were collected in 18 hours later and serum IL-1β level as a marker of inflammation was quantified. Also, liver histological assessment was performed to score the inflammatory grade. Results and Conclusion: In spite of dexamethasone group, both hydrodynamic/Ad-Luc and Ad-Luc receiving groups induced the expression of IL-1β cytokine (p<0.05). The pathological views also indicated more inflammation and necrosis inside the different zones of liver tissue in these two adenovector injected groups. These results emphasized that Adenovector served as a suitable inflammatory model and hydrodynamic injection enhances this kind of inflammation.
Keywords: Hydrodynamic injection, Adenoviral vector, Inflammation
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Type of Study: Original article | Subject: General
Received: 2015/05/22 | Accepted: 2015/05/22 | Published: 2015/05/22
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Rezaei F, Hosseini S Y, Pakniat A, Geramizadeh B, Fattahi M, Nejabat N et al . Adenovector Mediated Inflammation in Mice Liver Boosted by Hydrodynamic Injection. Iran J Virol 2013; 7 (3) :22-26
URL: http://journal.isv.org.ir/article-1-133-en.html

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