[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Contact ::
:: Volume 9, Issue 3 (8-2015) ::
Iran J Virol 2015, 9(3): 32-36 Back to browse issues page
Molecular Detection of Lentogenic Strain of Newcastle Disease Virus in Commercial Broiler Chickens Using Sentinel Birds in Iran: The First Report
Hossein Hosseini *, Amir Gholami, Arash Ghalyanchi Langeroudi
Department of Clinical Sciences, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran
Abstract:   (3793 Views)


Background and Aims: Newcastle disease (ND) is a highly contagious disease that affects many species of birds and causes significant economic losses to the poultry industry worldwide and the pathogenicity of Newcastle disease virus (NDV) strains varies with different virulence. We aim to detect lentogenic (low virulence) ND virus (ND/IR 2010) using sentinel birds and molecular detection system in commercial broiler farms in Iran as the first molecular case.

Materials and Methods: Partial sequencing of Fusion gene (F) which revealed that ND/IR 2010 carries the motif (112) GRQGRL (117) indicates that they are lentogenic genotype. Phylogenetic analysis, based on sequences of the F gene, revealed that our isolate should be classified as class II genotype I NDVs and related to the V4-like vaccine strain.

Results: Since the V4 vaccine strain is not used as vaccine in Iranian poultry industry, thus findings in this study are important and interesting. The results of alignment of  gene showed that ND/IR 2010 has 99.9% amino acid homology with V4, I2 and some other lentogenic NDV from South East Asia (in particular to duck origin virus).

Conclusions: This study adds to the understanding of the ecology of ND viruses in Iran and emphasizes the need for constant surveillance for more focus on lentogenic strains and their role in commercial farms and water fowl population as a neglected viral infection agent

Keywords: Newcastle Disease Virus, Iran, Lentogenic, Phylogenetic study
Full-Text [PDF 382 kb]   (1346 Downloads)    
Type of Study: Original article | Subject: General
Received: 2016/09/26 | Accepted: 2017/02/25 | Published: 2017/02/25
Send email to the article author

Add your comments about this article
Your username or Email:


XML     Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hosseini H, Gholami A, Ghalyanchi Langeroudi A. Molecular Detection of Lentogenic Strain of Newcastle Disease Virus in Commercial Broiler Chickens Using Sentinel Birds in Iran: The First Report. Iran J Virol 2015; 9 (3) :32-36
URL: http://journal.isv.org.ir/article-1-225-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 9, Issue 3 (8-2015) Back to browse issues page
Persian site map - English site map - Created in 0.05 seconds with 30 queries by YEKTAWEB 4549