[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Contact ::
:: Volume 5, Issue 1 (2-2011) ::
Iran J Virol 2011, 5(1): 10-14 Back to browse issues page
Characterization of Celery Mosaic Virus from Celery in Tehran Province
N Khoshkhatti *, M Habibi-Koohi, G Mosahebi
Department Plant Pathology, Faculty of Agricultural Science and Engineering, University of Tehran, College of Agriculture and Natural Resources, Karaj, Iran
Abstract:   (4213 Views)
Background and Aims: Celery mosaic virus (CeMV) is one of the causal agents of viral diseases in celery (Apium graveolens). CeMV is a member of the Potyvirus genus in Potyviridae family. The virus is naturally transmitted by aphids in a non-persistent manner. During growing season 2006-2007 viral disease symptoms were observed in celery fields grown in Tehran Province (Bage daneshkade, Mohamadshahr, Varamin, Asgarabad, Hesarak and Savojbolagh) and suspected CeMV infected plants were sampled. Methods: Serological assays with specific antisera against CeMV and other potyviruses revealed that 5.42% of the samples were infected with CeMV in Tehran province. Biologically purified CeMV isolate was mechanically inoculated on test plants for host range studies. Results: The virus caused chlorotic local lesions on Chenopodium quinoa and C. amaranticolor. It also produced mosaic, vein clearing and leaf deformations symptoms on Apium graveolens. Molecular mass of CeMV coat protein was estimated about 38.36 kDa using SDS-PAGE and was approved by western blotting using CeMV specific polyclonal antibody. A DNA fragment of about 650bp was amplified by RT-PCR using CeMV infected crude RNA and universal primer pair for potyviruses coat protein genome. Conclusion: This is the first study on this virus in Iran (Tehran Province).
Keywords: Celery Mosaic Virus, Serological assays, Province
Full-Text [PDF 311 kb]   (1495 Downloads)    
Type of Study: Original article | Subject: General
Received: 2014/11/7 | Accepted: 2014/11/7 | Published: 2014/11/7
Add your comments about this article
Your username or Email:


XML     Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Khoshkhatti N, Habibi-Koohi M, Mosahebi G. Characterization of Celery Mosaic Virus from Celery in Tehran Province. Iran J Virol 2011; 5 (1) :10-14
URL: http://journal.isv.org.ir/article-1-61-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 5, Issue 1 (2-2011) Back to browse issues page
Persian site map - English site map - Created in 0.05 seconds with 30 queries by YEKTAWEB 4514