[صفحه اصلی ]   [Archive] [ English ]  
:: صفحه اصلي :: درباره نشريه :: آخرين شماره :: تمام شماره‌ها :: جستجو :: ثبت نام :: ارسال مقاله :: تماس با ما ::
:: دوره 8، شماره 1 - ( 11-1392 ) ::
جلد 8 شماره 1 صفحات 25-32 برگشت به فهرست نسخه ها
An Investigation of Apple Chlorotic Leaf Spot (ACLSV) and Tomato Ring Spot (ToRSV) in Some Iranian Pear Gardens
چکیده:   (3022 مشاهده)

Background and Aims: In spite of a long tradition of fruit-tree growing in all provinces of Iran, information on pear (Pyrus communis) tree viruses is scant. In order to identify of the nature of the virus like symptoms and decline in pear trees, we carried out a survey for the occurrence of four important viruses.

Materials and Methods: Leaf samples of pear showing virus like symptoms were collected in three provinces, Iran, in 2013 and 2014 and tested for Apple chlorotic leaf spot (ACLSV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV) and Tomato ring spot (ToRSV) infection by ELISA, using specific antibodies.

Results: 39 out of 112 leaf samples were found to be infected by at least ToRSV and ACLSV. ToRSV was detected in the symptomatic samples from all surveyed provinces with infection ranging from 32.4% up to 35.0% however, the infection of ACLSV was differed between 9.9 to 18.2 %. Infections of ELISA positive samples were confirmed by biological assay. DNA fragments of 580 and 700 bp in size were RT-PCR amplified using specific primers designed according to RdRp and CP genes of ToRSV and ACLSV, respectively which indicated 96 and 92 % highest identities with available sequences of ToRSV and ACLSV isolates in GenBank, respectively.

Conclusion: This analysis provides, to our knowledge, the first demonstration of pear trees infection with ACLSV and ToRSV in Iran. Early detection of viral pathogens and accurate diagnosis, are undoubtedly essential for controlling the spread of virus and ensuring production of healthy plant reproductive material for further cultivation.

متن کامل [PDF 204 kb]   (1038 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: عمومى
دریافت: ۱۳۹۴/۹/۲۷ | پذیرش: ۱۳۹۴/۹/۲۷ | انتشار: ۱۳۹۴/۹/۲۷
ارسال نظر درباره این مقاله
نام کاربری یا پست الکترونیک شما:

CAPTCHA



XML   English Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Farmahini M, Pourrahim R, Elahinia A, Rouhibakhsh A, Farzadfar S. An Investigation of Apple Chlorotic Leaf Spot (ACLSV) and Tomato Ring Spot (ToRSV) in Some Iranian Pear Gardens . Iran J Virol. 2014; 8 (1) :25-32
URL: http://journal.isv.org.ir/article-1-168-fa.html

An Investigation of Apple Chlorotic Leaf Spot (ACLSV) and Tomato Ring Spot (ToRSV) in Some Iranian Pear Gardens . مجله ویروس شناسی ایران. 1392; 8 (1) :25-32

URL: http://journal.isv.org.ir/article-1-168-fa.html



دوره 8، شماره 1 - ( 11-1392 ) برگشت به فهرست نسخه ها
Persian site map - English site map - Created in 0.05 seconds with 32 queries by YEKTAWEB 3974