[صفحه اصلی ]   [Archive] [ English ]  
:: صفحه اصلي :: درباره نشريه :: آخرين شماره :: تمام شماره‌ها :: جستجو :: ثبت نام :: ارسال مقاله :: تماس با ما ::
:: دوره 4، شماره 1 - ( 4-1389 ) ::
جلد 4 شماره 1 صفحات 1-6 برگشت به فهرست نسخه ها
Development of a Recombinant Based ELISA using Specific Antibodies to F Protein in HCV Chronically Infected Patients-A Seroprevalence Study
چکیده:   (4335 مشاهده)

Background and Aims: The hepatitis C virus (HCV) F protein is a recently described, frameshift product of HCV core encoding sequence. Its function and antigenic properties are unknown. In order to assess the presence of antibodies specific for F protein we characterized specific anti-F antibodies in patients with chronic HCV infection.

Methods: The F protein was cloned from the HCV genome. The recombinant protein was expressed in Escherichia coli and purified by immunoaffinity chromatography. An enzyme-linked immunosorbent assay was developed using the purified recombinant HCV F protein.

Results: Serum samples were collected from 72 patients with chronic HCV infection and from 30 healthy controls. Eighty-two percent of chronic HCV patients had evidence of anti-F antibodies. 59 samples out of the 72 HCV infected patients exhibited a positive anti-F reaction, showing significant difference from the controls with no HCV infection (P < 0.01).

Conclusion: Based on these findings, HCV F protein elicits a specific antibody response, so frameshift could occur in the core-coding sequence in HCV genotype 1a in Iranian patients. As the first report, the prevalence of anti-F antibodies in chronic hepatitis C in Iran is of the order of 82%.

متن کامل [PDF 374 kb]   (649 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: عمومى
دریافت: ۱۳۹۳/۸/۱۶ | پذیرش: ۱۳۹۳/۸/۱۶ | انتشار: ۱۳۹۳/۸/۱۶
ارسال نظر درباره این مقاله
نام کاربری یا پست الکترونیک شما:

CAPTCHA



XML   English Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hashempour T, Ajorloo M, Bamdad T, Merat S, Zaer-Rezaee H, Fakharzadeh E, et al . Development of a Recombinant Based ELISA using Specific Antibodies to F Protein in HCV Chronically Infected Patients-A Seroprevalence Study. Iran J Virol. 2010; 4 (1) :1-6
URL: http://journal.isv.org.ir/article-1-46-fa.html

Development of a Recombinant Based ELISA using Specific Antibodies to F Protein in HCV Chronically Infected Patients-A Seroprevalence Study. مجله ویروس شناسی ایران. 1389; 4 (1) :1-6

URL: http://journal.isv.org.ir/article-1-46-fa.html



دوره 4، شماره 1 - ( 4-1389 ) برگشت به فهرست نسخه ها
Persian site map - English site map - Created in 0.06 seconds with 32 queries by YEKTAWEB 3974