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Complete Genomic Sequence of a Strain of Tomato Yellow Leaf Curl Virus from Iran
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چکیده: (4387 مشاهده) |
| Background and Aims: Tomato yellow leaf curl virus (TYLCV) is one of the most destructive viruses of tomato that leads to reduced tomato yield up to 100% in tropical and subtropical regions. In this study, the complete sequence of TYLCV isolate from Hormozgan province, Iran and its recombination evsent was determined. Methods: TYLCV infected tomato was collected from Hormozgan province. Total DNA was extracted from infected tomato and whiteflies and used as template for amplification by PCR. Analyses of sequences data were done by Clustal W method and GeneDoc software and then phylogenic tree and bootstrapping was prepared by the Maximum likelihood method by Clustal X with 100 replication. Amino acids analysis was carried out by neighbor joining method and RDP3 with 1000 replication. Results: A 670 bp fragment was amplified by using the specific primer for TYLCV and was then sequenced. Based on the 670 bp sequence, new primers were designed to amplify and characterize the next part in TYLCV circular genomeA 670 bp fragment was amplified by using the specific primer for TYLCV and was then sequenced. Based on the 670 bp sequence, new primers were designed to amplify and characterize the next part in TYLCV circular genome. Conclusion: Gene analysis showed that the genome includes six ORFs with 94% identity relatives which were most close to TYLCV-Kahnooj. It also showed that this isolate has an additional part of sequences in the rep gene that has not been found in the other strains of TYLCV. |
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متن کامل [PDF 434 kb]
(1339 دریافت)
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نوع مطالعه: پژوهشي |
موضوع مقاله:
عمومى
دریافت: 1393/8/16 | پذیرش: 1393/8/16 | انتشار: 1393/8/16
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