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:: دوره 6، شماره 2 - ( 2-1391 ) ::
جلد 6 شماره 2 صفحات 8-11 برگشت به فهرست نسخه ها
Cloning and Expression of Rabies Virus Glycoprotein Gene into Eukaryotic System
چکیده:   (3238 مشاهده)
Background and Aims: The aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcDNA3.1(+) in BSR cell line. This construct might be used for a potential DNA vaccine. Materials and Methods: Glycoprotein gene was synthesized and cloned into pBluescript vector and then sub cloned into eukaryotic expression vector (pcDNA3.1(+)). After verification of the cloning, the recombinant plasmid was transfected into BSR cell line (a clone of BHK-21 cell), and its expression was detected by RT-PCR. Results: The authenticity of the recombinant plasmid pcDNA3.1(+)-Gp has been confirmed by a quick check method and restriction endonuclease digestion analysis, and after transfection into eukaryotic cells, the presence of mRNA transcripts was verified by reverse transcriptase-polymerase chain reaction (RT-PCR). Conclusion: This study demonstrated that the construction of eukaryotic expression plasmid for rabies virus glycoprotein is possible. Nevertheless, more work is necessary to develop this kind of vaccine for final use.
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نوع مطالعه: پژوهشي | موضوع مقاله: عمومى
دریافت: ۱۳۹۳/۸/۱۶ | پذیرش: ۱۳۹۳/۸/۱۶ | انتشار: ۱۳۹۳/۸/۱۶
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Borhani K, Bamdad T, Ajorloo M, Mozhgani S, Miandehi N, Moradi-Joshaghan A et al . Cloning and Expression of Rabies Virus Glycoprotein Gene into Eukaryotic System. Iran J Virol. 2012; 6 (2) :8-11
URL: http://journal.isv.org.ir/article-1-90-fa.html

Cloning and Expression of Rabies Virus Glycoprotein Gene into Eukaryotic System. مجله ویروس شناسی ایران. 1391; 6 (2) :8-11

URL: http://journal.isv.org.ir/article-1-90-fa.html



دوره 6، شماره 2 - ( 2-1391 ) برگشت به فهرست نسخه ها
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