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:: Volume 6, Issue 2 (5-2012) ::
Iran J Virol 2012, 6(2): 8-11 Back to browse issues page
Cloning and Expression of Rabies Virus Glycoprotein Gene into Eukaryotic System
K Borhani , T Bamdad , M Ajorloo , SHR Mozhgani , N Miandehi , A Moradi-Joshaghan , AR Gholami
Department of Virology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abstract:   (2625 Views)
Background and Aims: The aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcDNA3.1(+) in BSR cell line. This construct might be used for a potential DNA vaccine. Materials and Methods: Glycoprotein gene was synthesized and cloned into pBluescript vector and then sub cloned into eukaryotic expression vector (pcDNA3.1(+)). After verification of the cloning, the recombinant plasmid was transfected into BSR cell line (a clone of BHK-21 cell), and its expression was detected by RT-PCR. Results: The authenticity of the recombinant plasmid pcDNA3.1(+)-Gp has been confirmed by a quick check method and restriction endonuclease digestion analysis, and after transfection into eukaryotic cells, the presence of mRNA transcripts was verified by reverse transcriptase-polymerase chain reaction (RT-PCR). Conclusion: This study demonstrated that the construction of eukaryotic expression plasmid for rabies virus glycoprotein is possible. Nevertheless, more work is necessary to develop this kind of vaccine for final use.
Keywords: Rabies virus, glycoprotein, DNA vaccine, Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)
Full-Text [PDF 148 kb]   (943 Downloads)    
Type of Study: Original article | Subject: General
Received: 2014/11/7 | Accepted: 2014/11/7 | Published: 2014/11/7
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Borhani K, Bamdad T, Ajorloo M, Mozhgani S, Miandehi N, Moradi-Joshaghan A et al . Cloning and Expression of Rabies Virus Glycoprotein Gene into Eukaryotic System. Iran J Virol. 2012; 6 (2) :8-11
URL: http://journal.isv.org.ir/article-1-90-en.html


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