:: دوره 4، شماره 1 - ( 4-1389 ) ::
جلد 4 شماره 1 صفحات 1-6 برگشت به فهرست نسخه ها
Development of a Recombinant Based ELISA using Specific Antibodies to F Protein in HCV Chronically Infected Patients-A Seroprevalence Study
چکیده:   (4879 مشاهده)

Background and Aims: The hepatitis C virus (HCV) F protein is a recently described, frameshift product of HCV core encoding sequence. Its function and antigenic properties are unknown. In order to assess the presence of antibodies specific for F protein we characterized specific anti-F antibodies in patients with chronic HCV infection.

Methods: The F protein was cloned from the HCV genome. The recombinant protein was expressed in Escherichia coli and purified by immunoaffinity chromatography. An enzyme-linked immunosorbent assay was developed using the purified recombinant HCV F protein.

Results: Serum samples were collected from 72 patients with chronic HCV infection and from 30 healthy controls. Eighty-two percent of chronic HCV patients had evidence of anti-F antibodies. 59 samples out of the 72 HCV infected patients exhibited a positive anti-F reaction, showing significant difference from the controls with no HCV infection (P < 0.01).

Conclusion: Based on these findings, HCV F protein elicits a specific antibody response, so frameshift could occur in the core-coding sequence in HCV genotype 1a in Iranian patients. As the first report, the prevalence of anti-F antibodies in chronic hepatitis C in Iran is of the order of 82%.

متن کامل [PDF 374 kb]   (718 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: عمومى
دریافت: 1393/8/16 | پذیرش: 1393/8/16 | انتشار: 1393/8/16



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دوره 4، شماره 1 - ( 4-1389 ) برگشت به فهرست نسخه ها