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Showing 3 results for Borhani
K Borhani , T Bamdad , M Ajorloo , Shr Mozhgani , N Miandehi , A Moradi-Joshaghan , Ar Gholami ,
Volume 6, Issue 2 (5-2012)
Abstract
Background and Aims: The aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcDNA3.1(+) in BSR cell line. This construct might be used for a potential DNA vaccine. Materials and Methods: Glycoprotein gene was synthesized and cloned into pBluescript vector and then sub cloned into eukaryotic expression vector (pcDNA3.1(+)). After verification of the cloning, the recombinant plasmid was transfected into BSR cell line (a clone of BHK-21 cell), and its expression was detected by RT-PCR. Results: The authenticity of the recombinant plasmid pcDNA3.1(+)-Gp has been confirmed by a quick check method and restriction endonuclease digestion analysis, and after transfection into eukaryotic cells, the presence of mRNA transcripts was verified by reverse transcriptase-polymerase chain reaction (RT-PCR). Conclusion: This study demonstrated that the construction of eukaryotic expression plasmid for rabies virus glycoprotein is possible. Nevertheless, more work is necessary to develop this kind of vaccine for final use.
Shr Mozhgani , E Faghihloo , M Makvandi , Ar Samarbaf-Zadeh , R Zareh-Khoshchehreh , M Ajorloo , Sd Mousavi-Nasab , K Borhani ,
Volume 6, Issue 3 (8-2012)
Abstract
NO abstract this article: Phylogenetic Analysis of Human Astrovirus Infection among Children Suffering from Gastroenteritis Referred to Aboozar Hospital, Ahvaz, Iran
Mr Ramtin Vamenani , H Rahimian, Sm Alavi, B Borhani ,
Volume 9, Issue 1 (2-2015)
Abstract
Abstract
Background and Aims: Apple scar skin disease (ASSD) is one of the destructive diseases of pome fruits which is caused by ASSVd.
Case presentation: We report molecular detection of ASSVd in symptomatic apples cv. Red Delicious with simultaneous presence of a new variant of HSVd from Khorasan Razavi province.
Conclusion: This is the first report on the association of HSVd with ASSVd in apple trees showing merely symptoms of apple dapple on the fruit.
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