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Showing 2 results for Morshed
H Hosseini, R Morshed ,
Volume 6, Issue 4 (11-2012)
Abstract
Background and Aims: Inclusion body hepatitis (IBH) is one of the most important reemerging diseases in many countries with intensive poultry industries. In Iran, the etiological agent of IBH (fowl adenovirus) has not been yet confirmed. The aim of this study was the molecular detection and identification of fowl adenovirus involving IBH in chicken flocks in Iran. Materials and Methods: Polymerase chain reaction (PCR) and sequence analysis of L1 hexon gene was utilized to detect and to determinate the genotypes of Fowl adenovirus (FAdV) in broiler breeder flocks. Histopathological sections were prepared and examined. Results: FAdVs were detected in livers. Based on sequencing analysis of the hexon gene, they were genetically related to FAdV-11, a member of the fowl adenovirus D species, with 98% homology to Korean strain in 2011. Histological examination revealed necrotizing hepatitis with basophilic intranuclear inclusion bodies in the hepatocytes. Conclusion: This study provided evidence for the role of fowl adenoviruses as agents, causing this clinical disease in Iran and indicated the importance of accurate diagnosis and prevention in the meat-type flocks.
Narges Mafigholami, Arash Ghalyanchilangeroudi, Hossein Hosseini, Hadi Haghbin Nazarpak, Rima Morshed,
Volume 15, Issue 1 (6-2021)
Abstract
Background and Aims: Enteric diseases have had detrimental impacts on commercial poultry through depressing growth rates and caused considerable economic losses to poultry producers. Chicken astrovirus (CAstV) is one of the most common viruses related to enteric diseases in chickens, especially in young chicks.
Materials and Methods: Chicken astroviruses were detected by reverse transcriptase and polymerase chain reaction (RT-PCR) in intestinal contents collected from commercial chickens. RT-PCR test amplified a fragment of 601 base pairs located in conserved regions within the ORF 1b (RNA polymerase) gene.
Results: Astroviruses were detected in birds from 36 poorly performing flocks with signs of enteric disease or retarded growth. In total, chicken astroviruses were discovered in pooled intestinal contents of 16 (44.4%) affected broiler flocks, while twenty flocks were negative (55.6%). Phylogenetic analysis based on a 601-bp segment of the ORF1b gene revealed two subgroups of Iranian astroviruses.
Conclusion: This study discloses the presence of chicken astroviruses in broiler chickens in Iran with enteric problems and stunting syndrome for the first time.
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