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:: Volume 15, Issue 1 (6-2021) ::
Iran J Virol 2021, 15(1): 45-49 Back to browse issues page
Evaluation of the Silymarin Effect on IL-10 and IL-17 Production in Chronic HCV Infected Patients
Pedram Attar Delgosha, Mehrdad Ravanshad * , Ali Maleki
Tarbiat Modares University
Abstract:   (299 Views)
Background and Aims: The use of herbal remedies, either in combination with conventional drugs or as an alternative, is accepted worldwide. Silymarin derived from Milk thistle has evidence-based therapeutic potency for wide spectrum of liver diseases. The current work aimed to study the immune-modulating activity of Silymarin in HCV-infected patients by measuring the effect of pure Silymarin solution on the production of IL-17 and IL-10.
Materials and Methods: Nine HCV-1a infected patients and three healthy controls were entered in this study. The mean age of patients and healthy controls were 45.53 (± 10.21) and 39.9 (±10.88), respectively. The PBMCs were isolated, cultured in 96-well plate and incubated with Silymarin solution (5µg/ml) for 24 hours. The cells and cell culture supernatant of three groups including patients treated with Silymarin, non-treated patients, and healthy controls were then subjected to Real Time PCR and ELISA to measure the levels of inflammatory and non-inflammatory cytokines including IL-17 and IL-10, respectively. Statistical analysis was conducted using SPSS software version 20.0.
Results: According to Real Time PCR and ELISA results, the level of IL-17 was significantly reduced in patients treated with Silymarin while the expression of IL-10 was remarkably increased in these patients.
Conclusion: The results of this study approved the immunomodulatory properties of Silymarin in HCV-infected patients. Hepatoprotective, antiviral, as well as immune-modulatory properties of the Silymarin make it a potential therapeutic option in patients with chronic hepatitis C.
Keywords: Silymarin, Hepatitis C Virus (HCV), Interleukin 10 (IL-10), Interleukin 17 (IL-17), Real Time PCR, ELISA
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Type of Study: Original article | Subject: General
Received: 2020/10/18 | Accepted: 2021/06/30 | Published: 2021/06/30
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Attar Delgosha P, Ravanshad M, Maleki A. Evaluation of the Silymarin Effect on IL-10 and IL-17 Production in Chronic HCV Infected Patients. Iran J Virol. 2021; 15 (1) :45-49
URL: http://journal.isv.org.ir/article-1-410-en.html

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Volume 15, Issue 1 (6-2021) Back to browse issues page
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