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Immunosuppression Viral Diseases in Healthy Broiler Flocks: The Pilot Study
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چکیده: (263 مشاهده) |
Abstract
Background and Aims: Immunosuppressive viral diseases have become a significant reason for mor-tality and economic losses in the poultry industry, mainly because of the increased susceptibility of to second bacterial infections and low responses to vaccination. The aim of this study, was to investigate the immunosuppressive viral agent, including IBDV, IBV, MDV, FadV, NDV, AIV, and CAV, in healthy farms. Bursas samples were collected from 3-4 week old broilers farm without clinical signs. One part of bursas fixed by immersion in 10% buffered formalin for histopathologic study and other parts selected for molecular studies. The RNA and DNA were extracted. PCR and RT-PCR were performed for this RNA and DNA viruses, respectively, and Phylogenetic analysis was conducted for IBD positive samples, also fixed tissue prepared for the histopathological purpose. Generally, four samples of 12 bursas samples were IBDV positive, five samples IBV positive (%41.66), and two samples MDV positive (16.66%).
Interestingly these four IBDV positive samples showed the histopathological changes described above. According to the results of PCR and histopathology determine that % 33.33 of broilers farm in Iran are suffering from subclinical IBD. All samples for AIV, NDV, CAV, and IBH were negative in PCR detection methods. The phylogenic tree analysis results show that these sequences are similar to other IBDV strains circulating in Iran broiler farms and belong to very virulent IBDV genogroups. |
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متن کامل [PDF 793 kb]
(111 دریافت)
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نوع مطالعه: پژوهشي |
موضوع مقاله:
تخصصي
دریافت: 1403/11/16 | پذیرش: 1402/4/15 | انتشار: 1402/4/15
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