[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Contact ::
Main Menu
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Contact us::
Site Facilities::
Medical Journalism::
Search in website

Advanced Search

:: Volume 5, Issue 2 (5-2011) ::
Iran J Virol 2011, 5(2): 1-8 Back to browse issues page
Herpes Simplex Virus Type 1 Latency-Associated Transcript Reduces Human Neuroblastoma Cell Proliferation
E Arefian , M Soleimani * , T Bamdad , Z Mobarra , A Hajarizadeh
Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abstract:   (4961 Views)
Background and Aims: The latency-associated transcript (LAT) transcribed by latent Herpes Simplex Virus type-1 in neuron cells are able to influence their host cell pathways. While the most of previous studies were focused on anti-apoptotic effects of LAT, our investigation is making an effort to explore LAT potency on cell cycle pathway in neuroblastoma cell lines. Methods: The evaluation of LAT expression was assayed by RT-PCR. Real-Time PCR of cell cycle critical gene controllers’ transcripts expression like EP300, P15, RB, RBL1, RBL2, MAPK-1, cyclinA2 and SMAD2 along with other technical evaluation such as MTT and cell counting assay assessed the LAT effects on cell cycle. Results: The LAT transfected cells gene expression showed the increase of EP300, P15, RBL1 and RBL2 along with decline in MAPK-1 and cyclinA2 in comparison to cells transfected by control vector. The cell counting and MTT assays determined that LAT brought cell cycle down rather than cells introduced by control plasmid. Conclusion: our investigation revealed that LAT transcript is able to repress cell cycle in neuroblastoma cells.
Keywords: Latency associated transcript, Herpes simplex virus type 1 (HSV-1), Cell cycle
Full-Text [PDF 300 kb]   (1085 Downloads)    
Type of Study: Original article | Subject: General
Received: 2014/11/7 | Accepted: 2014/11/7 | Published: 2014/11/7
Add your comments about this article
Your username or Email:


XML     Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Arefian E, Soleimani M, Bamdad T, Mobarra Z, Hajarizadeh A. Herpes Simplex Virus Type 1 Latency-Associated Transcript Reduces Human Neuroblastoma Cell Proliferation. Iran J Virol 2011; 5 (2) :1-8
URL: http://journal.isv.org.ir/article-1-65-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 5, Issue 2 (5-2011) Back to browse issues page
Persian site map - English site map - Created in 0.05 seconds with 39 queries by YEKTAWEB 4624