[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Contact ::
Main Menu
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Contact us::
Site Facilities::
Medical Journalism::
Search in website

Advanced Search

:: Volume 4, Issue 1 (7-2010) ::
Iran J Virol 2010, 4(1): 12-16 Back to browse issues page
Amantadine-Resistant among Seasonal H1N1 and 2009 Pandemic Isolated of Influenza A Viruses in Iran
M Ghaderi , T Mokhtari-Azad * , Sh Shahmahmoodi , K Samimi-Rad , J Yavarian , M Naseri , S Abbasi , M Norozbabaei , M Fazeli
National Influenza Center, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (4950 Views)
Background and Aims: Influenza A viruses are important pathogens for humans especially in pandemic episodes. Two adamantane derivates, amantadine and rimantadine, are used for prophylaxis and treatment of influenza A virus infections. However, single amino acid substitutions in the M2 transmembrane domain which lead to amantadine resistance of these viruses occur at residues 26, 27, 30, 31 or 34. Rates of resistant viruses have been increasing globally. Methods: In this report, 21 specimens of seasonal H1N1 and pandemic influenza A viruses which grew on MDCK cell line were studied for detection of amantadine resistant viruses. After RT-PCR M2 gene of samples were sequenced. In addition, as confirmatory assay, amplification of pandemic influenza A viruses on amantadine treated MDCK cell line and evaluation of TCID50 assay, were accomplished. Results: All seasonal influenza A viruses were amantadine sensitive but none of the 2009 pandemic influenza A viruses where us none of the 2009 pandemic influenza A virus were sensitive. Conclusion: Considering emergence of new influenza A virus variant, and resistance to amantadine, it is noteworthy that application of amantadine in new variant A/H1N1 influenza viruses might not be effective.
Keywords: Amantadine, influenza A virus, Iran
Full-Text [PDF 205 kb]   (1035 Downloads)    
Type of Study: Original article | Subject: General
Received: 2014/11/7 | Accepted: 2014/11/7 | Published: 2014/11/7
Add your comments about this article
Your username or Email:


XML     Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghaderi M, Mokhtari-Azad T, Shahmahmoodi S, Samimi-Rad K, Yavarian J, Naseri M, et al . Amantadine-Resistant among Seasonal H1N1 and 2009 Pandemic Isolated of Influenza A Viruses in Iran. Iran J Virol 2010; 4 (1) :12-16
URL: http://journal.isv.org.ir/article-1-48-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 4, Issue 1 (7-2010) Back to browse issues page
Persian site map - English site map - Created in 0.05 seconds with 39 queries by YEKTAWEB 4639