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:: Volume 8, Issue 2 And 3 (11-2014) ::
Iran J Virol 2014, 8(2 And 3): 26-35 Back to browse issues page
Incidence of Hepatitis B virus Surface Antigen (HBsAg) Mutations in Naïve Treated of Chronic Carriers from Tehran Metropolis, Iran
Hossein Keyvani , Fatemeh Ramezani, Seyem Mohamad Jazayeri, Seyed Hamid reza Monavari, Farah Bokharaei-Salim, Seyed Moayed Alavian, Ghodrat Montazeri, Mehdi Norouzi, Maryam Daram, Abolfazl Khedive, Reza Malekzadeh, Mohamad Amin Pourhoseingholi, Reza Rezaee, Lars Magnius, Helen Norder
Department of virology, Iran University of Medical Sciences, Tehran, Iran
Abstract:   (2285 Views)

Abstract Background: Naturally occurred hepatitis B virus (HBV) with surface mutations in a variety of chronic hepatitis B (CHB) patients who have received no vaccine or HBIG bearing substitutions in surface protein, have been reported. Objectives: Current knowledge concerning the prevalence of these naturally occurring surface antigen mutations among Iranian carriers is limited. Patients and Methods: In a retrospective cross-sectional study, 119 inactive HBV chronic carriers were enrolled. The surface gene was amplified, sequenced and subsequently aligned using international and national sequence database. Results: All strains belonged to genotype D, subgenotype D1 and subtype ayw2. In 74 (62.18%) of patients, 146 (68.8%) out of 212 amino acid mutations occurred in different immune epitopes within surface protein, of which 28 (19.17%) in B cell, 37 (25.34%) in T helper and 81 (55.47%) inside CTL epitopes. 13 (8.9%) and 15 (10.27%) of amino acid substitutions occurred outside and within the “a” determinant in Major Hydrophilic Region (MHR). While 11 (9.24%) and 77 (64.7%) patients who harbored amino acid mutations, were HBeAg and anti-HBe positive, respectively (p=0.004). 9 and 63 amino acid mutations were occurred in different HBsAg epitopes in HBeAg and anti-HBe positive patients, respectively (P=0.04). Conclusions: HBV mutants within the surface immune epitopes seem to be extremely common among chronic carriers from Tehran, especially those who are anti-HBe positive, indicating that after HBeAg seroconversion, due to the selection pressure of e antibody, the occurrence of mutation is an inevitable effect of the evolutionary process.

Keywords: HBsAg mutations, HBV genotype D, HBV Iran, HBsAg immune epitopes
Full-Text [PDF 352 kb]   (495 Downloads)    
Type of Study: Original article | Subject: Special
Received: 2015/07/31 | Accepted: 2016/01/17 | Published: 2016/01/17
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Keyvani H, Ramezani F, Jazayeri S M, Monavari S H R, Bokharaei-Salim F, Alavian S M, et al . Incidence of Hepatitis B virus Surface Antigen (HBsAg) Mutations in Naïve Treated of Chronic Carriers from Tehran Metropolis, Iran. Iran J Virol. 2014; 8 (2 and 3) :26-35
URL: http://journal.isv.org.ir/article-1-141-en.html


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