[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Contact ::
:: Volume 8, Issue 4 (2-2014) ::
Iran J Virol 2014, 8(4): 1-5 Back to browse issues page
Evaluation of the Role of Herpes Simplex Virus Type 1 in the Secretion of Interleukin-1β (IL-1β) by Cultured Gingival Epithelial Cells
S Yaghobee, A Khorsand, H Safari, A Ataei-Pirkooh, HR Monavvari *
Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
Abstract:   (4316 Views)

Background and Aims: Several studies have shown that there is greater number of viruses in deep periodontal pockets than in normal gingival sulcus. We also know that virus may have an important role in induction of Il-1β secretion by different human cells. In this study we intended to determine the capacity of Herpes simplex virus type 1 to stimulate Il-1β secretion in human gingival keratinocytes.

Materials and Methods: primary gingival keratinocytes were obtained from samples extracted during crown lengthening surgical procedure. The cells were cultured in 24 well flasks and stimulated by one of these stimulants: LPS, HSV1, LPS+HSV1 and LPS+ATP. After incubation and collecting the supernatants, the amount of secreted Il-1β was measured by ELISA technique.

Results: none of the stimulants were able to cause significant amount of Il-1β secretion in any of the incubation times.  

Conclusion: It seems that HSV1 does not have the capacity to induct IL-1β secretion as a single factor alone or in combination with bacterial LPS.

Keywords: Inflammasome, HSV1, IL-1b, Lipopolysaccharide, gingival keratinocytes
Full-Text [PDF 318 kb]   (1243 Downloads)    
Type of Study: Original article | Subject: Special
Received: 2016/02/17 | Accepted: 2016/02/17 | Published: 2016/02/17
Send email to the article author

Add your comments about this article
Your username or Email:


XML     Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Yaghobee S, Khorsand A, Safari H, Ataei-Pirkooh A, Monavvari H. Evaluation of the Role of Herpes Simplex Virus Type 1 in the Secretion of Interleukin-1β (IL-1β) by Cultured Gingival Epithelial Cells. Iran J Virol 2014; 8 (4) :1-5
URL: http://journal.isv.org.ir/article-1-177-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 8, Issue 4 (2-2014) Back to browse issues page
Persian site map - English site map - Created in 0.04 seconds with 30 queries by YEKTAWEB 4570