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:: Volume 9, Issue 2 (5-2015) ::
Iran J Virol 2015, 9(2): 14-19 Back to browse issues page
HHV-6 DNA and Antibody Detection in Plasma and PBMC of Multiple Sclerosis Patients in North of Iran
Akramsadat Ahmadi, Mohammad Hasan Naeimi, Naeme Javid, Abdolvahab Moradi, Mohammad Ali Vakili, Alijan Tabarraei *
Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran
Abstract:   (4175 Views)

Abstract

Background and Aims: Multiple sclerosis (MS) is defined by the presence of inflammatory demyelinating plaques in the central nervous system (CNS). It has been indicated that human herpes virus 6 (HHV-6) may play role in pathogenesis of MS. The aim of this study was to determine the presence of HHV-6 DNA and anti HHV-6 IgG in MS patients and controls.

Materials and Methods: Blood samples from 59 patients with MS and 59 healthy controls were collected. DNA extraction and real-time PCR based on (Syber Green) were done in the peripheral blood mononuclear cells (PBMCs) and in plasma specimens. Anti-HHV-6 IgG was measured by ELISA technique. Demographic and clinical data were collected and entered in SPSS 16 and analyzed.

Results: HHV-6 DNA was found in the plasma sample of 1 (1.7%) of 59 MS patients. There was no HHV-6 DNA in PBMCs of the MS patients and controls and in plasma of control group. Anti- HHV-6 IgG antibody was present in 84.7% of MS patients and 82.1% of control group.

Conclusion: There was no significant relation based on presence of HHV-6 DNA and antibody between MS patients and controls in north of Iran.

Keywords: HHV-6, Multiple sclerosis, Real-Time PCR, ELISA
Full-Text [PDF 151 kb]   (1436 Downloads)    
Type of Study: Original article | Subject: Special
Received: 2016/05/17 | Accepted: 2016/07/5 | Published: 2016/09/5
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Ahmadi A, Naeimi M H, Javid N, Moradi A, Vakili M A, Tabarraei A. HHV-6 DNA and Antibody Detection in Plasma and PBMC of Multiple Sclerosis Patients in North of Iran. Iran J Virol. 2015; 9 (2) :14-19
URL: http://journal.isv.org.ir/article-1-184-en.html


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