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Iran J Virol 2015, 9(4): 9-17 Back to browse issues page
Cytomegalovirus PCR Analysis of Serum and Ocular Swab Samples in Patients with Inflammatory Eye Disease
Jalil Omidian, Fariba Sheikhi-Shooshtari
Department of Ophthalmology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
Abstract:   (3064 Views)

Abstract

Background and Aims: Cytomegalovirus (CMV) infection is very common in the population. Virus belongs to the family Herpesviridae, whose representatives are characterized by the ability to cause the human body's latent persistent infections. The goal of the study is to assess the CMVV infection frequency and PCR method in the choice of CMV in inflammatory eye disease, Comparing with CMV presence in Eye Infection and the control group.

Materials and Methods: Primers were designed for conserved regions of the CMV genome. We have used PCR to rapid, accurate detection of EBV DNA in blood and from eye swabs as well as pp65 antigenemia. We have chosen to study patients with eye inflammation or infection symptom.

Results: CMV DNA was detectable in three 21 out of 130 control samples of serum (16.5%). CMV infection was seen in 9 out of 20 (45%) patients’ serum samples. Compared with the controls, the presence of EBV DNA was only significantly increased in samples of the patient group. 13 out of 20 (65%) patients in patients and 19 out of 130 (14.61%) of the control group had detectable CMV DNA in their ocular swab.

Conclusion: We have presented evidence for the presence of CMV sequences in normal and eye inflammation samples with PCR. CMV serology was available for a large number of individuals. The prevalence of CMV in ocular disease samples varied dramatically that this wide range may be due to variations and inconsistency in the techniques used to detect the virus and its Components, as well as geography and genetic susceptibility.

Keywords: cytomegalovirus, CMV, PCR analysis, serum and ocular swab, inflammatory eye disease
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Type of Study: Original article | Subject: General
Received: 2016/10/16 | Accepted: 2016/10/16 | Published: 2016/10/16
  • CMV serology was available for a large number of individuals.
  • The prevalence of CMV in ocular disease samples varied dramatically that this wide range may be due to variations and inconsistency in the techniques used to detect the virus and its Components, as well as geography and genetic susceptibility.

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Omidian J, Sheikhi-Shooshtari F. Cytomegalovirus PCR Analysis of Serum and Ocular Swab Samples in Patients with Inflammatory Eye Disease. Iran J Virol. 2015; 9 (4) :9-17
URL: http://journal.isv.org.ir/article-1-235-en.html


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