|
Design and Fabrication of Rapid Diagnostic Test Based on Gold Nanosensors to Detect SARS-CoV-2
|
Angila Ataei-Pirkooh   , Omid Pourdakan , Ahmad Tavakoli , Alireza Ghasempour , Vahid Pirhajati-Mahabadi , Alireza Mohebbi , Bita Mehravi * |
| Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran |
|
|
Abstract: (330 Views) |
Abstract
Background and Aims: Early detection of COVID-19 can decrease the motality rate. The diagnostic tools available so far have been based on a) detection of viral genes, b) detection of human antibodies and c) detection of viral antigens, among which detection of viral genes by RT-PCR is recognized as the most reliable method. In order to speed up the detection process and reduce the false negative results presented, it is necessary to design an accurate and fast test.
Methods: A specific probe against N gene was designed and then gold nanoparticles were synthesized by citrate reduction method. The properties of the synthesized Nano systems were determined in terms of size, surface charge, morphology and accuracy of nanoparticle formation using dynamic light scattering device, zeta potential, transmission electron microscope and ultraviolet light spectroscopy. The designed probe was added to gold nanoparticles after activation. Negative and positive samples were taken from patients and added to nanoparticles containing probes.
Results: Due to the free the sulfhydryl group and the high bond energy between the gold and this group, after the probe was added to the nanoparticles, change in color was observed in the nanoparticle solution. UV Spectrum and DLS results showed 4 nm red shift and change in size from 11 to 16 nm, respectively. In the presence of the SARS-CoV-2 RNA, AuNPs-probe would bound with RNA particles, resulting in a space barrier against salt particle on the other hand in negative samples, the salt bridge would be formed and agglomeration of AuNPs and a change in color would be observed by naked eye. |
|
| Keywords: Biosensor, Colorimetric detection, Gold Nanoparticles, Rapid test, SARS-CoV-2 |
|
|
Full-Text [PDF 546 kb]
(123 Downloads)
|
Type of Study: Original article |
Subject:
Special
Received: 2022/07/31 | Accepted: 2024/12/29 | Published: 2023/06/21
|
|
|
|
|
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
.jpeg)
