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Molecular Detection of Human Enteroviruses in Wastewater Treatment Plant
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Z Azarash   , M Esghaei , M Farzadkia , F Bokharaei-Salim , SJ Kiani , SH Monavari , G Mokhtary-Irani , MS Hosseini , A Ataei-Pirkooh * |
| Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. |
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Abstract: (1128 Views) |
Background and Aims: Enteroviruses are spread worldwide that cause many diseases, including gastrointestinal infections. These viruses are considered as an important pathogen in wastewater and therefore their presence in treated wastewater can cause disease transmission. We aimed to investigate the molecular prevalence and characterization of human enteroviruses in wastewater samples of a wastewater treatment in Tehran, Iran.
Materials and Methods: From November 2017 to April 2018, a total of 15 samples were collected by Grab sampling method from three chambers including the chamber adjacent to the aeration tank and the pre-chlorination effluent and the post-chlorination effluent. The samples were analysed for the presence of human enteroviruses by an integrated cell culture/polymerase chain reaction (ICC/PCR) technique. Then, the isolated enteroviruses were evaluated using sequencing analysis.
Results: The results showed that the rate of infection with enteroviruses using culture method and RT-PCR technique in wastewater samples was 80% and 40%, respectively. Also, human enteroviruses in the samples were Coxsackievirus B5 (50%), poliovirus type 1 vaccine (33%) and Echovirus 11 (16%).
Conclusion: Human enteroviruses were detected in wastewater samples and the highest rate with coxsackievirus B5 (50%) and then with poliovirus type 1 vaccine (33%) and Echovirus 11 (16%). |
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| Keywords: Wastewater, ICC-PCR, Human Enteroviruses |
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Full-Text [PDF 411 kb]
(532 Downloads)
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Type of Study: Original article |
Subject:
Special
Received: 2022/08/1 | Accepted: 2020/06/25 | Published: 2020/06/25
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