|
Investigation of the Relationship between Virus Neutralization Test and ELISA with Challenge Test in Evaluation of FMD Vaccine Potency
|
Mahdieh Akbarzadegan   , Homayoon Mahravani *  , Zeinab Piravar |
| Foot and Mouth Disease Reference Laboratory, Razi Vaccine & Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran. institute. Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran. |
|
|
Abstract: (85 Views) |
Abstract
Background and Aims: Foot-and-mouth disease (FMD), is a highly infectious and contagious disease in livestock. An effective and efficient vaccine is needed to Control FMD and reduces the associated damage. The goal of this study was to find out if the protective dose or challenge (PD50%) could be used instead of the antibody titer method to measure the effectiveness of the FMD vaccine. To achieve this, several calves were selected and divided into four groups. The vaccine was administered in three doses: a full dose, a 1/3 dose and a 1/9 dose. Twenty-one days after vaccination, all animals were challenged with a 10,000 LID50% virus in the tongue epithelium. For 7 days after, the animals were evaluated and monitored for the appearance of FMD symptoms. The PD50% (Protective Dose) for each vaccine against the virus was determined in the experiment. After obtaining the VN results that indicated the antibody titer and the PD50% level, a comparison was made between these two parameters. By examining 5 test cases, a formula was derived that accurately determined the PD50% with a high degree of precision using the VN50% result. This study determined the constants for A and O types using the VN50% test results. By incorporating these values into the derived formula, the PD50% level could be determined. |
|
| Keywords: FMD, serum neutralization test, inactivated FMD vaccine, challenge test, determination of protective dose. |
|
|
Full-Text [PDF 469 kb]
(33 Downloads)
|
Type of Study: Original article |
Subject:
Special
Received: 2023/08/29 | Accepted: 2024/09/24 | Published: 2023/07/19
|
|
|
|
|
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
.jpeg)
