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:: Volume 6, Issue 3 (8-2012) ::
Iran J Virol 2012, 6(3): 19-23 Back to browse issues page
Development of a Multiplex Polymerase Chain Reaction for Differential Diagnosis of Canary Pox Virus
MM Ebrahimi , S Shahsavandi, S Masoudi, N Ghodsian, A Hashemi, MH Hablalvarid, AR Hatami
Razi vaccine & serum Research Institute, Karaj, Iran
Abstract:   (3446 Views)
Background and Aims: A multiplex transcription-polymerase chain reaction (m-PCR) was developed for direct detection and discrimination between canarypox virus (CPV) and other avian poxvirus (APV). Materials and Methods: Three compatible primer sets were designed for m-PCR amplification of different loci fpv126, fpv140, and fpv167 located at highly conserved APV genes. Results: Results showed that m-PCR products of the expected sizes were obtained for all of the primer sets when they were tested either alone or in combination with an artificial mixture of positive controls. Based on the better amplification of fpv167 than other loci, the locus primer set was used to examine tissue samples from canaries clinically diagnosed as AVP-infected. Conclusion: All canary samples were positive for CPV by the m-PCR and virus isolation. The results of the present study indicate the m-PCR assay holds potential to be versatile, rapid, and sensitive for detection of CPV and differentiation of the virus from the other APVs.
Keywords: Canary pox virus, Fragment-Length Polymorphism, Diagnosis
Full-Text [PDF 391 kb]   (1204 Downloads)    
Type of Study: Original article | Subject: General
Received: 2014/11/7 | Accepted: 2014/11/7 | Published: 2014/11/7
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Ebrahimi M, Shahsavandi S, Masoudi S, Ghodsian N, Hashemi A, Hablalvarid M et al . Development of a Multiplex Polymerase Chain Reaction for Differential Diagnosis of Canary Pox Virus. Iran J Virol. 2012; 6 (3) :19-23
URL: http://journal.isv.org.ir/article-1-98-en.html


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