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:: Volume 13, Issue 2 (12-2019) ::
Iran J Virol 2019, 13(2): 24-28 Back to browse issues page
Detection of Avian Encephalomyelitis Virus in Broiler Chickens in Iran Using RT-PCR and Histopathological Methods
Mohammadreza Ghorani, Arash Ghalyanchi Langeroudi , Ali Akramian, Hossein Hosseini, Fatah Rohani
Microbiology and Immunology Department, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Abstract:   (425 Views)
Introduction: Avian encephalomyelitis is caused by a Tremovirus and primarily affects chickens. The virus can infect young chicks and cause nervous symptoms. Vaccines are used to control the disease in breeders. Recently, the occurrence of the disease is causing concern in the poultry industry.
Materials and Methods: In the present study, we report a case of avian encephalomyelitis in one broiler farm in Kashan (center of Iran). The chickens showed nervous symptoms like depression, head trembling, ataxia, dull eyes, as well as drowsiness, lack of coordination, and unsteady gait in chickens. In this study, we detected avian encephalomyelitis with the molecular procedure like reverse transcriptase-polymerase chain reaction (RT-PCR). Also, the histopathological diagnosis was made.  
Results: The disease was confirmed based on the clinical, molecular and histopathological findings.
Conclusions: Due to the increase in vaccine prices and the difficulties of vaccine providing in the breeders’ farms, the probability of disease has increased. Stringently monitoring of breeders farms before the beginning of egg production and using a suitable protocol with vaccination is recommended.   
Keywords: avian encephalomyelitis, broiler, RT-PCR, histopathology, Iran
Full-Text [PDF 273 kb]   (44 Downloads)    
Type of Study: Original article | Subject: Special
Received: 2020/02/7 | Accepted: 2020/04/15 | Published: 2020/04/15
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Ghorani M, Ghalyanchi Langeroudi A, Akramian A, Hosseini H, Rohani F. Detection of Avian Encephalomyelitis Virus in Broiler Chickens in Iran Using RT-PCR and Histopathological Methods. Iran J Virol. 2019; 13 (2) :24-28
URL: http://journal.isv.org.ir/article-1-374-en.html


Volume 13, Issue 2 (12-2019) Back to browse issues page
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