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Study of Fibroblast Growth Factor 3 (fgf-3) Gene Expression in Breast Cancer Tissues Infected with Mouse Mammary Tumor Virus (MMTV)
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Hassan Vahidi Emami   , Arash Ghalyanchi Langeroudi * , Seyed Masoud Hosseini , Hamideh Najafi |
| Department of Microbiology and Immunology, School of the Veterinary Medicine, University of Tehran, Tehran, Iran. |
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Abstract: (404 Views) |
Abstract
Background and Aims: Breast cancer (BC) is the second most common cancer among women worldwide. Several factors can influence the occurrence of BC, including the presence of murine mammary tumor virus (MMTV). In this study, we aimed to perform the epidemiology of MMTV and investigation of human fgf-3 gene expression in cancerous and noncancerous individuals infected with MMTV
Materials and Methods: A total of 85 BC tissue samples and 85 tumor-free margin tissue samples (normal tissue) were collected from women undergoing breast surgery at general hospitals in the Qom Province, Iran. The presence of the MMTV env gene was determined using the nested PCR method. Additionally, the correlation between the fgf-3 gene expression and the MMTV-env gene conducted by Real-time PCR for the first time in Iran.
Results: The MMTV env gene was detected in 16 (18.8%) out of 85 BC tissue samples, but in only 2 (2.35%) samples without cancer. In the MMTV-positive samples, the expression of the fgf-3 gene was increased compared to MMTV-negative samples (P=0.012). Furthermore, there was no significant difference in fgf-3 gene expression between grade I and II BC samples. However, a significant increase in fgf-3 gene expression was observed in grade III and TNBC cases. (P< 0.05).
Conclusion: The mouse mammary tumor virus represents a significant risk factor for BC. Therefore, implementing preventive measures, including timely MMTV infection detection and control, can be effective in BC prevention programs in Iran and globally. |
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| Keywords: MMTV, Breast cancer, fgf-3 gene, Real-time PCR method. |
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Full-Text [PDF 569 kb]
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Type of Study: Original article |
Subject:
Special
Received: 2025/02/4 | Accepted: 2023/07/10 | Published: 2023/07/10
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