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:: Volume 5, Issue 4 (11-2011) ::
Iran J Virol 2011, 5(4): 1-5 Back to browse issues page
Comparison of Passive Haemagglutination and Neutralization Tests to Demonstrate Genital Herpes Virus (HSV2) Antibodies in Pregnant Women
M Nejati, H Soleimanjahi , MH Roostaee, B Pourhossein
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abstract:   (2670 Views)
Background and Aims: Herpes simplex virus (HSV) is responsible for several significant human viral diseases, with severity ranging from subclinical to fatal infection. Herpes simplex virus type 2 (HSV2) infections are more commonly seen in association with the genitalia and surrounding areas, and can be transmitted to newborns during childbirth. Generalized infections in newborns are also predominantly HSV2. Therefore, due to the increasing HSV2 infections especially subclinical in women, the need to diagnose herpes simplex virus infections has increased. Materials and Methods: In the present study, the passive haemagglutination (PHA) test was applied to determine the titer of Anti-HSV2 antibodies. Sheep red blood cells (SRBCs) were treated with tannic acid at concentration of 0.002% and sensitized with HSV2 that had been propagated in HeLa cells. The tannic acid treated and HSV2 sensitized SRBCs were added to U-shaped 96 wells microtiter plates which contained serial dilutions of patient’s sera. Furthermore, serum neutralization test (SNT) was applied as a gold standard to determine the specificity and sensitivity of PHA test. Results: The results of PHA were examined after one hour incubation at 37°C. The endpoint was the highest dilution of serum which gave positive agglutination and compaired with serum neutralization test (SNT as a gold standard to determine the specificity and sensitivity of PHA test. Peripheral blood samples were obtained from 100 pregnant women and evaluated by both SNT and PHA tests. The specificity and sensitivity of PHA test were 92.68%, and 100% respectively. The results indicated that PHA test was easy, rapid and inexpensive but also had acceptable sensitively and specificity. Conclusion: PHA test can be used to determine the level of Anti-HSV2 antibodies and the rate of infection in pregnant women which could be an indication of infection and the risk of transmitting herpes to the newborn.
Keywords: Herpes Simplex virus type 2, passive haemagglutination test, serum neutralization test
Full-Text [PDF 309 kb]   (463 Downloads)    
Type of Study: Original article | Subject: General
Received: 2014/11/7 | Accepted: 2014/11/7 | Published: 2014/11/7
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Nejati M, Soleimanjahi H, Roostaee M, Pourhossein B. Comparison of Passive Haemagglutination and Neutralization Tests to Demonstrate Genital Herpes Virus (HSV2) Antibodies in Pregnant Women. Iran J Virol. 2011; 5 (4) :1-5
URL: http://journal.isv.org.ir/article-1-77-en.html


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