|
Cloning and Expression of Rabies Virus Glycoprotein Gene into Eukaryotic System
|
K Borhani   , T Bamdad * , M Ajorloo , SHR Mozhgani , N Miandehi , A Moradi-Joshaghan , AR Gholami |
| Department of Virology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran |
|
|
Abstract: (6302 Views) |
| Background and Aims: The aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcDNA3.1(+) in BSR cell line. This construct might be used for a potential DNA vaccine. Materials and Methods: Glycoprotein gene was synthesized and cloned into pBluescript vector and then sub cloned into eukaryotic expression vector (pcDNA3.1(+)). After verification of the cloning, the recombinant plasmid was transfected into BSR cell line (a clone of BHK-21 cell), and its expression was detected by RT-PCR. Results: The authenticity of the recombinant plasmid pcDNA3.1(+)-Gp has been confirmed by a quick check method and restriction endonuclease digestion analysis, and after transfection into eukaryotic cells, the presence of mRNA transcripts was verified by reverse transcriptase-polymerase chain reaction (RT-PCR). Conclusion: This study demonstrated that the construction of eukaryotic expression plasmid for rabies virus glycoprotein is possible. Nevertheless, more work is necessary to develop this kind of vaccine for final use. |
|
| Keywords: Rabies virus, glycoprotein, DNA vaccine, Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) |
|
|
Full-Text [PDF 148 kb]
(2581 Downloads)
|
Type of Study: Original article |
Subject:
General
Received: 2014/11/7 | Accepted: 2014/11/7 | Published: 2014/11/7
|
|
|
|
|
|
|
| Add your comments about this article |
|
|
|
.jpeg)
